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    Authors: Midgley P.A.1; Weyland M.. 3D imaging, electron tomography, transmission electron microscopy (TEM), scanning transmission electron microscopy. We used electron tomography (29, 30) to produce (3D) reconstructions of desmosomes in newborn mouse epidermis. Because samples of skin are. We report a new variant of (3D) electron microscopy, Z-contrast tomography, in which, by use of a field-emission gun scanning

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